• Western blot analysis of extracts of various cell lines, using GLUT3/SLC2A3 Antibody (STJ110449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of GLUT3/SLC2A3 in paraffin-embedded rat testis using GLUT3/SLC2A3 Rabbit polyclonal antibody (STJ110449) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using GLUT3/SLC2A3 rabbit polyclonal antibody (STJ110449) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GLUT3/SLC2A3 in paraffin-embedded rat brain using GLUT3/SLC2A3 Rabbit polyclonal antibody (STJ110449) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat testis using GLUT3/SLC2A3 rabbit polyclonal antibody (STJ110449) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using GLUT3/SLC2A3 Rabbit polyclonal antibody (STJ110449) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SLC2A3 antibody (207-281) (STJ110449)

SKU:
STJ110449

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLUT3 (207-281) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC2A3
Gene ID: 6515
Uniprot ID: GTR3_HUMAN
Immunogen Region: 207-281
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 207-281 of human GLUT3/SLC2A3 (NP_008862.1).
Immunogen Sequence: ESPRFLLINRKEEENAKQIL QRLWGTQDVSQDIQEMKDES ARMSQEKQVTVLELFRVSSY RQPIIISIVLQLSQQ
Tissue Specificity Highly expressed in brain. Expressed in many tissues.
Function Facilitative glucose transporter. Can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport. Required for mesendoderm differentiation.
Protein Name Solute Carrier Family 2 - Facilitated Glucose Transporter Member 3
Glucose Transporter Type 3 - Brain
Glut-3
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-196836
Reactome: R-HSA-6798695
Reactome: R-HSA-9022699
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Perikaryon
Cell Projection
Localized To Densely Spaced Patches Along Neuronal Processes
Alternative Antibody Names Anti-Solute Carrier Family 2 - Facilitated Glucose Transporter Member 3 antibody
Anti-Glucose Transporter Type 3 - Brain antibody
Anti-Glut-3 antibody
Anti-SLC2A3 antibody
Anti-GLUT3 antibody

Information sourced from Uniprot.org

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