Immunofluorescence analysis of U2OS cells using SLC12A6 Rabbit pAb (STJ117853) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SLC12A6 antibody (60-190) (STJ117853)

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STJ117853
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: IF/ICC/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC12A6
Gene ID: 9990
Uniprot ID: S12A6_HUMAN
Immunogen Region: 60-190
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 60-190 of human SLC12A6 (NP_598408.1).
Immunogen Sequence: MSEMSGATTSLATVALDPPS DRTSHPQDVIEDLSQNSITG EHSQLLDDGHKKARNAYLNN SNYEEGDEYFDKNLALFEEE MDTRPKVSSLLNRMANYTNL TQGAKEHEEAENITEGKKKP TKTPQMGTFMG
Tissue Specificity Expressed in brain (at protein level). Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2: More abundant in kidney. Isoform 5: Testis specific.
Post Translational Modifications Phosphorylated, phosphorylation regulates transporter activity. Phosphorylated at Thr-991 and Thr-1048 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), inhibiting the potassium-chloride cotransport activity. N-glycosylated.
Function Isoform 1: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. Isoform 2: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 3: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 4: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 5: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable). Isoform 6: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable).
Protein Name Solute Carrier Family 12 Member 6
Electroneutral Potassium-Chloride Cotransporter 3
K-Cl Cotransporter 3
Database Links Reactome: R-HSA-426117
Reactome: R-HSA-5619039
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 6 antibody
Anti-Electroneutral Potassium-Chloride Cotransporter 3 antibody
Anti-K-Cl Cotransporter 3 antibody
Anti-SLC12A6 antibody
Anti-KCC3 antibody

Information sourced from Uniprot.org

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