• Western blot analysis of extracts of various cell lines, using SHMT1 antibody (STJ11105690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SHMT1 antibody (285-444) (STJ11105690)

SKU:
STJ11105690

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine Hydroxymethyltransferase-Cytosolic (285-444) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHMT1
Gene ID: 6470
Uniprot ID: GLYC_HUMAN
Immunogen Region: 285-444
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).
Immunogen Sequence: FKVYQHQVVANCRALSEALT ELGYKIVTGGSDNHLILVDL RSKGTDGGRAEKVLEACSIA CNKNTCPGDRSALRPSGLRL GTPALTSRGLLEKDFQKVAH FIHRGIELTLQIQSDTGVRA TLKEFKERLAGDKYQAAVQA LREEVESFASLFPLPGLPDF
Function Interconversion of serine and glycine.
Protein Name Serine Hydroxymethyltransferase - Cytosolic
Shmt
Glycine Hydroxymethyltransferase
Serine Methylase
Database Links Reactome: R-HSA-196757
Reactome: R-HSA-71262
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Serine Hydroxymethyltransferase - Cytosolic antibody
Anti-Shmt antibody
Anti-Glycine Hydroxymethyltransferase antibody
Anti-Serine Methylase antibody
Anti-SHMT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance