• Immunofluorescence analysis of Hep G2 cells using SHMT1 Rabbit polyclonal antibody (STJ110038) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded Human kidney tissue using SHMT1 Rabbit polyclonal antibody (STJ110038) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of SHMT1 in paraffin-embedded rat brain using SHMT1 Rabbit polyclonal antibody (STJ110038) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SHMT1 in paraffin-embedded rat kidney using SHMT1 Rabbit polyclonal antibody (STJ110038) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SHMT1 in paraffin-embedded human stomach using SHMT1 Rabbit polyclonal antibody (STJ110038) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SHMT1 Rabbit polyclonal antibody (STJ110038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SHMT1 antibody (285-444) (STJ110038)

SKU:
STJ110038

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SHMT1 (285-444) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SHMT1
Gene ID: 6470
Uniprot ID: GLYC_HUMAN
Immunogen Region: 285-444
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).
Immunogen Sequence: FKVYQHQVVANCRALSEALT ELGYKIVTGGSDNHLILVDL RSKGTDGGRAEKVLEACSIA CNKNTCPGDRSALRPSGLRL GTPALTSRGLLEKDFQKVAH FIHRGIELTLQIQSDTGVRA TLKEFKERLAGDKYQAAVQA LREEVESFASLFPLPGLPDF
Function Interconversion of serine and glycine.
Protein Name Serine Hydroxymethyltransferase - Cytosolic
Shmt
Glycine Hydroxymethyltransferase
Serine Methylase
Database Links Reactome: R-HSA-196757
Reactome: R-HSA-71262
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Serine Hydroxymethyltransferase - Cytosolic antibody
Anti-Shmt antibody
Anti-Glycine Hydroxymethyltransferase antibody
Anti-Serine Methylase antibody
Anti-SHMT1 antibody

Information sourced from Uniprot.org

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