• Western blot analysis of extracts of various cell lines, using SGSH antibody (STJ110447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of Mouse lung, using SGSH antibody (STJ110447) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SGSH antibody (273-502) (STJ110447)

SKU:
STJ110447

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SGSH
Gene ID: 6448
Uniprot ID: SPHM_HUMAN
Immunogen Region: 273-502
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 273-502 of human SGSH (NP_000190.1).
Immunogen Sequence: DNGIPFPSGRTNLYWPGTAE PLLVSSPEHPKRWGQVSEAY VSLLDLTPTILDWFSIPYPS YAIFGSKTIHLTGRSLLPAL EAEPLWATVFGSQSHHEVTM SYPMRSVQHRHFRLVHNLNF KMPFPIDQDFYVSPTFQDLL NRTTAGQPTGWYKDLRHYYY RARWELYDRSRDPHETQNLA TDPRFAQLLEMLRDQLAKWQ WETHDPWVCAPDGVLEEKLS PQCQPLHNEL
Post Translational Modifications The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
Function Catalyzes a step in lysosomal heparan sulfate degradation.
Protein Name N-Sulphoglucosamine Sulphohydrolase
Sulfoglucosamine Sulfamidase
Sulphamidase
Database Links Reactome: R-HSA-2024096
Reactome: R-HSA-2206307
Cellular Localisation Lysosome
Alternative Antibody Names Anti-N-Sulphoglucosamine Sulphohydrolase antibody
Anti-Sulfoglucosamine Sulfamidase antibody
Anti-Sulphamidase antibody
Anti-SGSH antibody
Anti-HSS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance