Western blot analysis of extracts of various cell lines, using SGK2 antibody (STJ114030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SGK2 antibody (228-367) (STJ114030)

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STJ114030
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SGK2
Gene ID: 10110
Uniprot ID: SGK2_HUMAN
Immunogen Region: 228-367
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 228-367 of human SGK2 (NP_733794.1).
Immunogen Sequence: LHGLPPFYSQDVSQMYENIL HQPLQIPGGRTVAACDLLQS LLHKDQRQRLGSKADFLEIK NHVFFSPINWDDLYHKRLTP PFNPNVTGPADLKHFDPEFT QEAVSKSIGCTPDTVASSSG ASSAFLGFSYAPEDDDILDC
Tissue Specificity Highly expressed in liver, kidney and pancreas, and at lower levels in brain.
Post Translational Modifications Activated by phosphorylation on Ser-356 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-193.
Function Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na(+) channels: SCNN1A/ENAC, K(+) channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase.
Protein Name Serine/Threonine-Protein Kinase Sgk2
Serum/Glucocorticoid-Regulated Kinase 2
Database Links Reactome: R-HSA-2672351
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Sgk2 antibody
Anti-Serum/Glucocorticoid-Regulated Kinase 2 antibody
Anti-SGK2 antibody

Information sourced from Uniprot.org

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