• Western blot analysis of extracts of various cell lines, using SF3A2 antibody (STJ112708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SF3A2 antibody (1-220) (STJ112708)

SKU:
STJ112708

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SF3A2 (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3A2
Gene ID: 8175
Uniprot ID: SF3A2_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SF3A2 (NP_009096.2).
Immunogen Sequence: MDFQHRPGGKTGSGGVASSS ESNRDRRERLRQLALETIDI NKDPYFMKNHLGSYECKLCL TLHNNEGSYLAHTQGKKHQT NLARRAAKEAKEAPAQPAPE KVKVEVKKFVKIGRPGYKVT KQRDSEMGQQSLLFQIDYPE IAEGIMPRHRFMSAYEQRIE PPDRRWQYLLMAAEPYETIA FKVPSREIDKAEGKFWTHWN RETKQFFLQFHFKMEKPPAP
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes, including the Bact complex. Interacts directly with the duplex formed by U2 snRNA and the intron.
Protein Name Splicing Factor 3a Subunit 2
Sf3a66
Spliceosome-Associated Protein 62
Sap 62
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 3a Subunit 2 antibody
Anti-Sf3a66 antibody
Anti-Spliceosome-Associated Protein 62 antibody
Anti-Sap 62 antibody
Anti-SF3A2 antibody
Anti-SAP62 antibody

Information sourced from Uniprot.org

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