| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-SEH1L (260-360) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:1000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SEH1L |
| Gene ID: | 81929 |
| Uniprot ID: | SEH1_HUMAN |
| Immunogen Region: | 260-360 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-360 of human SEH1L (NP_112493.2). |
| Immunogen Sequence: | SGGPTKFEIHIVAQFDNHNS QVWRVSWNITGTVLASSGDD GCVRLWKANYMDNWKCTGIL KGNGSPVNGSSQQGTSNPSL GSTIPSLQNSLNGSSAGRKH S |
| Function | Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore. As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway. The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex. GATOR2 probably acts as a E3 ubiquitin-protein ligase toward GATOR1. In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation. In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex. Within the GATOR2 complex, SEC13 and SEH1L are required to stabilize the complex. |
| Protein Name | Nucleoporin Seh1Gator2 Complex Protein Seh1Nup107-160 Subcomplex Subunit Seh1Sec13-Like Protein |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-141444 Q96EE3-1Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-2467813 Q96EE3-1Reactome: R-HSA-2500257 Q96EE3-1Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-5663220 Q96EE3-1Reactome: R-HSA-6784531Reactome: R-HSA-68877 Q96EE3-1Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9615933 Q96EE3-1Reactome: R-HSA-9639288Reactome: R-HSA-9648025 Q96EE3-1Reactome: R-HSA-9705671 |
| Cellular Localisation | ChromosomeCentromereKinetochoreNucleusNuclear Pore ComplexLysosome Membrane |
| Alternative Antibody Names | Anti-Nucleoporin Seh1 antibodyAnti-Gator2 Complex Protein Seh1 antibodyAnti-Nup107-160 Subcomplex Subunit Seh1 antibodyAnti-Sec13-Like Protein antibodyAnti-SEH1L antibodyAnti-SEC13L antibodyAnti-SEH1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")