• Western blot analysis of extracts of various cell lines, using RRP7A antibody (STJ116363) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using RRP7A Rabbit polyclonal antibody (STJ116363) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RRP7A antibody (1-280) (STJ116363)

SKU:
STJ116363

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RRP7A (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRP7A
Gene ID: 27341
Uniprot ID: RRP7A_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RRP7A (NP_056518.2).
Immunogen Sequence: MVARRRKCAARDPEDRIPSP LGYAAIPIKFSEKQQASHYL YVRAHGVRQGTKSTWPQKRT LFVLNVPPYCTEESLSRLLS TCGLVQSVELQEKPDLAESP KESRSKFFHPKPVPGFQVAY VVFQKPSGVSAALALKGPLL VSTESHPVKSGIHKWISDYA DSVPDPEALRVEVDTFMEAY DQKIAEEEAKAKEEEGVPDE EGWVKVTRRGRRPVLPRTEA ASLRVLERERRKRSRKELL
Function Nucleolar protein that is involved in ribosomal RNA (rRNA) processing. Also plays a role in primary cilia resorption, and cell cycle progression in neurogenesis and neocortex development. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Ribosomal Rna-Processing Protein 7 Homolog A
Gastric Cancer Antigen Zg14
Database Links Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Cell Projection
Cilium
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Alternative Antibody Names Anti-Ribosomal Rna-Processing Protein 7 Homolog A antibody
Anti-Gastric Cancer Antigen Zg14 antibody
Anti-RRP7A antibody
Anti-CGI-96 antibody

Information sourced from Uniprot.org

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