• Western blot analysis of various lysates, using RNF125 Rabbit polyclonal antibody (STJ117359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RNF125 antibody (41-232) (STJ117359)

SKU:
STJ117359

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF125 (41-232) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF125
Gene ID: 54941
Uniprot ID: RN125_HUMAN
Immunogen Region: 41-232
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 41-232 of human RNF125 (NP_060301.2).
Immunogen Sequence: LEVLHQPVRTRCGHVFCRSC IATSLKNNKWTCPYCRAYLP SEGVPATDVAKRMKSEYKNC AECDTLVCLSEMRAHIRTCQ KYIDKYGPLQELEETAARCV CPFCQRELYEDSLLDHCITH HRSERRPVFCPLCRLIPDEN PSSFSGSLIRHLQVSHTLFY
Tissue Specificity Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4(+) and CD8(+) T-cells, suggesting that it is preferentially confined to T-cells.
Post Translational Modifications Autoubiquitinated, leading to its subsequent proteasomal degradation.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation.
Protein Name E3 Ubiquitin-Protein Ligase Rnf125
Ring Finger Protein 125
T-Cell Ring Activation Protein 1
Trac-1
Database Links Reactome: R-HSA-936440
Cellular Localisation Golgi Apparatus Membrane
Lipid-Anchor
Shows A Reticular Staining Pattern Within The Cell And Is Probably Expressed At Other Intracellular Membranes In Addition To The Golgi Membrane
Not Detected At The Plasma Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf125 antibody
Anti-Ring Finger Protein 125 antibody
Anti-T-Cell Ring Activation Protein 1 antibody
Anti-Trac-1 antibody
Anti-RNF125 antibody

Information sourced from Uniprot.org

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