• Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using RGL3 Rabbit polyclonal antibody (STJ11104874) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RGL3 antibody (1-106 aa) (STJ11104874)

SKU:
STJ11104874

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Host: Rabbit
Applications: WB/ELISA
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-106 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of Arabidopsis thaliana RGL3. (NP_197251.1).
Immunogen Sequence: MKRSHQETSVEEEAPSMVEK LENGCGGGGDDNMDEFLAVL GYKVRSSDMADVAQKLEQLE MVLSNDIASSSNAFNDTVHY NPSDLSGWAQSMLSDLNYYP DLDPNR
Background Encodes a DELLA subfamily member that acts as a negative regulator of GA signaling and as a coactivator of ABI3 to promote seed storage protein biosynthesis during the seed maturation stage. Involved in hyperosmotic salinity response, jasmonic acid mediated signaling pathway, negative regulation of gibberellic acid mediated signaling pathway, negative regulation of seed germination, regulation of DNA-templated transcription, regulation of reactive oxygen species metabolic process, regulation of seed dormancy process, response to abscisic acid, response to ethylene, salicylic acid mediated signaling pathway.

Information sourced from Uniprot.org

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