• Western blot analysis of extracts of various cell lines, using RDH10 antibody (STJ114029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RDH10 antibody (27-293) (STJ114029)

SKU:
STJ114029

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RDH10 (27-293) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RDH10
Gene ID: 157506
Uniprot ID: RDH10_HUMAN
Immunogen Region: 27-293
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-293 of human RDH10 (NP_742034.1).
Immunogen Sequence: VRPKEKSVAGQVCLITGAGS GLGRLFALEFARRRALLVLW DINTQSNEETAGMVRHIYRD LEAADAAALQAGNGEEEILP HCNLQVFTYTCDVGKRENVY LTAERVRKEVGEVSVLVNNA GVVSGHHLLECPDELIERTM MVNCHAHFWTTKAFLPTMLE INHGHIVTVASSLGLFSTAG VEDYCASKFGVVGFHESLSH ELKAAEKDGIKTTLVCPYLV DTGMFRGCRIRKEIEPFLP
Tissue Specificity Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle.
Function Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol.
Protein Name Retinol Dehydrogenase 10
Short Chain Dehydrogenase/Reductase Family 16c Member 4
Database Links Reactome: R-HSA-2453902
Reactome: R-HSA-5365859
Cellular Localisation Microsome Membrane
Single-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Retinol Dehydrogenase 10 antibody
Anti-Short Chain Dehydrogenase/Reductase Family 16c Member 4 antibody
Anti-RDH10 antibody
Anti-SDR16C4 antibody
Anti-UNQ9375 antibody
Anti-PRO34191 antibody

Information sourced from Uniprot.org

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