| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RCCD1 (230-360) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RCCD1 |
| Gene ID: |
91433 |
| Uniprot ID: |
RCCD1_HUMAN |
| Immunogen Region: |
230-360 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 230-360 of human RCCD1 (NP_001017919.1). |
| Immunogen Sequence: |
DIYIWGWNESGQLALPTRNL AEDGETVAREATELNEDGSQ VKRTGGAEDGAPAPFIAVQP FPALLDLPMGSDAVKASCGS RHTAVVTRTGELYTWGWGKY GQLGHEDTTSLDRPRRVEYF VDKQLQVKAVT |
| Post Translational Modifications | Specifically hydroxylated (with R stereochemistry) at C-3 of ARG-141 by KDM8. |
| Function | Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with KDM8. Possibly together with KDM8, is involved in proper mitotic spindle organization and chromosome segregation. Plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial to mesenchymal transition (EMT), potentially through the inhibition of KDM8. |
| Protein Name | Rcc1 Domain-Containing Protein 1 |
| Cellular Localisation | ChromosomeColocalizes With Trimethylated 'Lys-9' Of Histone H3 (H3k9me3) |
| Alternative Antibody Names | Anti-Rcc1 Domain-Containing Protein 1 antibodyAnti-RCCD1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89103/357560/STJ11100031_1__14868.1713121685.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
