• Western blot analysis of extracts from normal (control) and RAB1B knockout (KO) HeLa cells, using RAB1B antibody (STJ11100811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RAB1B antibody (7-201) (STJ11100811)

SKU:
STJ11100811

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB1B (7-201) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB1B
Gene ID: 81876
Uniprot ID: RAB1B_HUMAN
Immunogen Region: 7-201
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1).
Immunogen Sequence: YLFKLLLIGDSGVGKSCLLL RFADDTYTESYISTIGVDFK IRTIELDGKTIKLQIWDTAG QERFRTITSSYYRGAHGIIV VYDVTDQESYANVKQWLQEI DRYASENVNKLLVGNKSDLT TKKVVDNTTAKEFADSLGIP FLETSAKNATNVEQAFMTMA AEIKKRMGPGAASGGERPNL KIDSTPVKPAGGGCC
Post Translational Modifications Prenylated.by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). (Microbial infection) AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. It is later de-AMPylated by L.pneumophila SidD, releasing RAB1B from bacterial phagosomes. (Microbial infection) Phosphocholinated at Ser-76 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. (Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment.
Protein Name Ras-Related Protein Rab-1b
Database Links Reactome: R-HSA-162658
Reactome: R-HSA-204005
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cytoplasm
Membrane
Lipid-Anchor
Cytoplasmic Side
Preautophagosomal Structure Membrane
Perinuclear Region
Targeted By Rep1 To Membranes Of Specific Subcellular Compartments Including Endoplasmic Reticulum
Golgi Apparatus
And Intermediate Vesicles Between These Two Compartments
In The Gdp-Form
Colocalizes With Gdi In The Cytoplasm
Co-Localizes With Mtmr6 To The Endoplasmic Reticulum-Golgi Intermediate Compartment And To The Peri-Golgi Region
Alternative Antibody Names Anti-Ras-Related Protein Rab-1b antibody
Anti-RAB1B antibody

Information sourced from Uniprot.org

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