• Western blot analysis of extracts of A-549 cells, using RAB14 antibody (STJ114625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Neuro-2a cells, using RAB14 antibody (STJ114625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RAB14 antibody (39-140) (STJ114625)

SKU:
STJ114625

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB14 (39-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB14
Gene ID: 51552
Uniprot ID: RAB14_HUMAN
Immunogen Region: 39-140
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 39-140 of human RAB14 (NP_057406.2).
Immunogen Sequence: DCPHTIGVEFGTRIIEVSGQ KIKLQIWDTAGQERFRAVTR SYYRGAAGALMVYDITRRST YNHLSSWLTDARNLTNPNTV IILIGNKADLEAQRDVTYEE AK
Function Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes. Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion.
Protein Name Ras-Related Protein Rab-14
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1660499
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Recycling Endosome
Early Endosome Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Cytoplasmic Vesicle
Phagosome
Recruited To Recycling Endosomes By Dennd6a
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Alternative Antibody Names Anti-Ras-Related Protein Rab-14 antibody
Anti-RAB14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance