• Western blot analysis of lysates from wild type (WT) and PSIP1 knockout (KO) 293T cells, using [KO Validated] PSIP1 Rabbit polyclonal antibody (STJ11100074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PSIP1 antibody (301-530) (STJ11100074)

SKU:
STJ11100074

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSIP1 (301-530) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSIP1
Gene ID: 11168
Uniprot ID: PSIP1_HUMAN
Immunogen Region: 301-530
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 301-530 of human PSIP1 (NP_150091.2).
Immunogen Sequence: NMLKGQHEKEAADRKRKQEE QMETEQQNKDEGKKPEVKKV EKKRETSMDSRLQRIHAEIK NSLKIDNLDVNRCIEALDEL ASLQVTMQQAQKHTEMITTL KKIRRFKVSQVIMEKSTMLY NKFKNMFLVGEGDSVITQVL NKSLAEQRQHEEANKTKDQG KKGPNKKLEKEQTGSKTLNG GSDAQDGNQPQHNGESNEDS KDNHEASTKKKPSSEERETE ISLKDSTLDN
Tissue Specificity Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
Post Translational Modifications Citrullinated by PADI4.
Function Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
Protein Name Pc4 And Sfrs1-Interacting Protein
Cll-Associated Antigen Kw-7
Dense Fine Speckles 70 Kda Protein
Dfs 70
Lens Epithelium-Derived Growth Factor
Transcriptional Coactivator P75/P52
Database Links Reactome: R-HSA-162592
Reactome: R-HSA-164843
Reactome: R-HSA-175567
Reactome: R-HSA-177539
Reactome: R-HSA-180689
Reactome: R-HSA-180910
Reactome: R-HSA-9772755
Cellular Localisation Nucleus
Remains Chromatin-Associated Throughout The Cell Cycle
Alternative Antibody Names Anti-Pc4 And Sfrs1-Interacting Protein antibody
Anti-Cll-Associated Antigen Kw-7 antibody
Anti-Dense Fine Speckles 70 Kda Protein antibody
Anti-Dfs 70 antibody
Anti-Lens Epithelium-Derived Growth Factor antibody
Anti-Transcriptional Coactivator P75/P52 antibody
Anti-PSIP1 antibody
Anti-DFS70 antibody
Anti-LEDGF antibody
Anti-PSIP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance