| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1500-1:3000 IHC-P:1:500-1:2000 IF/ICC:1:200-1:800 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | DLG4 |
| Gene ID: | 1742 |
| Uniprot ID: | DLG4_HUMAN |
| Immunogen Region: | 1-100 aa |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PSD95 (NP_001308004.1). |
| Immunogen Sequence: | MDCLCIVTTKKYRYQDEDTP PLEHSPAHLPNQANSPPVIV NTDTLEAPGYELQVNGTEGE MEYEEITLERGNSGLGFSIA GGTDNPHIGDDPSIFITKII |
| Tissue Specificity | Brain. |
| Post Translational Modifications | Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses. Palmitoylation by ZDHHC2 occurs when the synaptic activity decreases and induces DLG4 synaptic clustering. Palmitoylation by ZDHHC15 regulates trafficking to the postsynaptic density and function in synaptogenesis. Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering. Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycles during neuronal development which slow down in mature neurons. Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis. |
| Function | Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression. Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins. |
| Protein Name | Disks Large Homolog 4 Postsynaptic Density Protein 95 Psd-95 Synapse-Associated Protein 90 Sap-90 Sap90 |
| Database Links | Reactome: R-HSA-1236394 Reactome: R-HSA-399719 Reactome: R-HSA-438066 Reactome: R-HSA-442982 Reactome: R-HSA-447038 Reactome: R-HSA-451308 Reactome: R-HSA-5625900 Reactome: R-HSA-5673001 Reactome: R-HSA-5682910 Reactome: R-HSA-6794361 Reactome: R-HSA-8849932 Reactome: R-HSA-9609736 Reactome: R-HSA-9617324 Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Cytoplasmic Side Postsynaptic Density Synapse Cytoplasm Cell Projection Axon Dendritic Spine Dendrite Presynapse High Levels In Postsynaptic Density Of Neurons In The Forebrain Also In Presynaptic Region Of Inhibitory Synapses Formed By Cerebellar Basket Cells On Axon Hillocks Of Purkinje Cells Suppression Of Neuronal Activity Induces Synaptic Accumulation And Clustering Of Dlg4 |
| Alternative Antibody Names | Anti-Disks Large Homolog 4 antibody Anti-Postsynaptic Density Protein 95 antibody Anti-Psd-95 antibody Anti-Synapse-Associated Protein 90 antibody Anti-Sap-90 antibody Anti-Sap90 antibody Anti-DLG4 antibody Anti-PSD95 antibody |
Information sourced from Uniprot.org