• Western blot analysis of lysates from HeLa cells, using PRPF18 Rabbit polyclonal antibody (STJ114683) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRPF18 antibody (1-342) (STJ114683)

SKU:
STJ114683

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRPF18 (1-342) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRPF18
Gene ID: 8559
Uniprot ID: PRP18_HUMAN
Immunogen Region: 1-342
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human PRPF18 (NP_003666.1).
Immunogen Sequence: MDILKSEILRKRQLVEDRNL LVENKKYFKRSELAKKEEEA YFERCGYKIQPKEEDQKPLT SSNPVLELELAEEKLPMTLS RQEVIRRLRERGEPIRLFGE TDYDAFQRLRKIEILTPEVN KGLRNDLKAALDKIDQQYLN EIVGGQEPGEEDTQNDLKVH EENTTIEELEALGESLGKGD DHKDMDIITKFLKFLLGVWA KELNAREDYVKRSVQGKLNS ATQKQTESYLRPLFRKLRK
Function Participates in the second step of pre-mRNA splicing.
Protein Name Pre-Mrna-Splicing Factor 18
Prp18 Homolog
Hprp18
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus Speckle
Colocalizes With Spliceosomal Snrnps
Alternative Antibody Names Anti-Pre-Mrna-Splicing Factor 18 antibody
Anti-Prp18 Homolog antibody
Anti-Hprp18 antibody
Anti-PRPF18 antibody
Anti-HPRP18 antibody

Information sourced from Uniprot.org

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