• Western blot analysis of extracts of various cell lines, using PRICKLE2 antibody (STJ110329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of A549 cells using PRICKLE2 antibody (STJ110329).
  • Immunofluorescence analysis of A549 cells using PRICKLE2 antibody (STJ110329).
  • Western blot analysis of extracts of various cell lines, using PRICKLE2 antibody (STJ110329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRICKLE2 antibody (615-844) (STJ110329)

SKU:
STJ110329

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRICKLE2 (615-844) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRICKLE2
Gene ID: 166336
Uniprot ID: PRIC2_HUMAN
Immunogen Region: 615-844
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 615-844 of human PRICKLE2 (NP_942559.1).
Immunogen Sequence: QEMEGNLHQLSNPIGYRDLQ SHGRMHQSFDFDGGMAGSKL PGQEGVRIQPMSERTRRRAT SRDDNRRFRPHRSRRSRRSR SDNALHLASEREAISRLKDR PPLRAREDYDQFMRQRSFQE SMGHGSRRDLYGQCPRTVSD LALQNAFGDRWGPYFAEYDW CSTCSSSSESDNEGYFLGEP IPQPARLRYVTSDELLHKYS SYGLPKSSTLGGRGQLHSRK RQKSKNCIIS
Tissue Specificity Expressed in brain, eye and testis. Additionally in fetal brain, adult cartilage, pancreatic islet, gastric cancer and uterus tumors.
Protein Name Prickle-Like Protein 2
Cellular Localisation Nucleus Membrane
Alternative Antibody Names Anti-Prickle-Like Protein 2 antibody
Anti-PRICKLE2 antibody

Information sourced from Uniprot.org

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