| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-POLI (50-350) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLI |
| Gene ID: |
11201 |
| Uniprot ID: |
POLI_HUMAN |
| Immunogen Region: |
50-350 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human POLI (NP_009126.2). |
| Immunogen Sequence: |
ASSRVIVHVDLDCFYAQVEM ISNPELKDKPLGVQQKYLVV TCNYEARKLGVKKLMNVRDA KEKCPQLVLVNGEDLTRYRE MSYKVTELLEEFSPVVERLG FDENFVDLTEMVEKRLQQLQ SDELSAVTVSGHVYNNQSIN LLDVLHIRLLVGSQIAAEMR EAMYNQLGLTGCAGVASNKL LAKLVSGVFKPNQQTVLLPE SCQHLIHSLNHIKEIPGIGY KTAKCLEALGINSVRDLQT |
| Tissue Specificity | Ubiquitous. Highly expressed in testis. |
| Post Translational Modifications | Monoubiquitinated. Protein monoubiquitination prevents POLI binding to ubiquitin via the ubiquitin-binding motif 1 and ubiquitin-binding motif 2. |
| Function | Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. |
| Protein Name | Dna Polymerase IotaEta2Rad30 Homolog B |
| Database Links | Reactome: R-HSA-5656121Reactome: R-HSA-5656169 |
| Cellular Localisation | NucleusBinding To Ubiquitin Mediates Localization To Replication Forks After Uv-Induced Dna Damage |
| Alternative Antibody Names | Anti-Dna Polymerase Iota antibodyAnti-Eta2 antibodyAnti-Rad30 Homolog B antibodyAnti-POLI antibodyAnti-RAD30B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
