• Western blot analysis of extracts of various cell lines, using POLD1 antibody (STJ27276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using POLD1 antibody (STJ27276). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A549 cells using POLD1 antibody (STJ27276).
  • Immunofluorescence analysis of U2OS cells using POLD1 antibody (STJ27276). Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

Anti-POLD1 antibody (808-1107) (STJ27276)

SKU:
STJ27276

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLD1 (808-1107) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLD1
Gene ID: 5424
Uniprot ID: DPOD1_HUMAN
Immunogen Region: 808-1107
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2).
Immunogen Sequence: RYAGLLFSSRPDAHDRMDCK GLEAVRRDNCPLVANLVTAS LRRLLIDRDPEGAVAHAQDV ISDLLCNRIDISQLVITKEL TRAASDYAGKQAHVELAERM RKRDPGSAPSLGDRVPYVII SAAKGVAAYMKSEDPLFVLE HSLPIDTQYYLEQQLAKPLL RIFEPILGEGRAEAVLLRGD HTRCKTVLTGKVGGLLAFAK RRNCCIGCRTVLSHQGAVCE FCQPRESELYQKEVSHLNA
Tissue Specificity Widely expressed, with high levels of expression in heart and lung.
Function As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites.
Protein Name Dna Polymerase Delta Catalytic Subunit
3'-5' Exodeoxyribonuclease
Dna Polymerase Subunit Delta P125
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-174411
Reactome: R-HSA-174414
Reactome: R-HSA-174417
Reactome: R-HSA-174437
Reactome: R-HSA-2564830
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-69091
Reactome: R-HSA-69166
Reactome: R-HSA-69183
Cellular Localisation Nucleus
Colocalizes With Pcna And Pold3 At S Phase Replication Sites
After Uv Irradiation
Recruited To Dna Damage Sites Within 2 Hours
Independently On The Cell Cycle Phase
Nor On Pcna Ubiquitination
This Recruitment Requires Pold3
Pcna And Rfc1-Replication Factor C Complex
Alternative Antibody Names Anti-Dna Polymerase Delta Catalytic Subunit antibody
Anti-3'-5' Exodeoxyribonuclease antibody
Anti-Dna Polymerase Subunit Delta P125 antibody
Anti-POLD1 antibody
Anti-POLD antibody

Information sourced from Uniprot.org

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