| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-POLA2 (1-250) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:3000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLA2 |
| Gene ID: |
23649 |
| Uniprot ID: |
DPOA2_HUMAN |
| Immunogen Region: |
1-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POLA2 (NP_002680.2). |
| Immunogen Sequence: |
MSASAQQLAEELQIFGLDCE EALIEKLVELCVQYGQNEEG MVGELIAFCTSTHKVGLTSE ILNSFEHEFLSKRLSKARHS TCKDSGHAGARDIVSIQELI EVEEEEEILLNSYTTPSKGS QKRAISTPETPLTKRSVSTR SPHQLLSPSSFSPSATPSQK YNSRSNRGEVVTSFGLAQGV SWSGRGGAGNISLKVLGCPE ALTGSYKSMFQKLPDIREVL TCKIEELGSELKEHYKIEA |
| Post Translational Modifications | Phosphorylated in a cell cycle-dependent manner, in G2/M phase. |
| Function | Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. |
| Protein Name | Dna Polymerase Alpha Subunit BDna Polymerase Alpha 70 Kda Subunit |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-174411Reactome: R-HSA-174430Reactome: R-HSA-68952Reactome: R-HSA-68962Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-9710421 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Polymerase Alpha Subunit B antibodyAnti-Dna Polymerase Alpha 70 Kda Subunit antibodyAnti-POLA2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
