| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PMS2 (390-670) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PMS2 |
| Gene ID: |
5395 |
| Uniprot ID: |
PMS2_HUMAN |
| Immunogen Region: |
390-670 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 390-670 of human PMS2 (NP_000526.2). |
| Immunogen Sequence: |
ADLEKPMVEKQDQSPSLRTG EEKKDVSISRLREAFSLRHT TENKPHSPKTPEPRRSPLGQ KRGMLSSSTSGAISDKGVLR PQKEAVSSSHGPSDPTDRAE VEKDSGHGSTSVDSEGFSIP DTGSHCSSEYAASSPGDRGS QEHVDSQEKAPKTDDSFSDV DCHSNQEDTGCKFRVLPQPT NLATPNTKRFKKEEILSSSD ICQKLVNTQDMSASQVDVAV KINKKVVPLDFSMSSLAKR |
| Function | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair. |
| Protein Name | Mismatch Repair Endonuclease Pms2Dna Mismatch Repair Protein Pms2Pms1 Protein Homolog 2 |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5545483Reactome: R-HSA-5632987Reactome: R-HSA-6796648 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mismatch Repair Endonuclease Pms2 antibodyAnti-Dna Mismatch Repair Protein Pms2 antibodyAnti-Pms1 Protein Homolog 2 antibodyAnti-PMS2 antibodyAnti-PMSL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
