• Western blot analysis of various lysates using PIGT Rabbit polyclonal antibody (STJ114013) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PIGT antibody (260-460) (STJ114013)

SKU:
STJ114013

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIGT (260-460) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGT
Gene ID: 51604
Uniprot ID: PIGT_HUMAN
Immunogen Region: 260-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIGT (NP_057021.2).
Immunogen Sequence: LFRMFSRTLTEPCPLASESR VYVDITTYNQDNETLEVHPP PTTTYQDVILGTRKTYAIYD LLDTAMINNSRNLNIQLKWK RPPENEAPPVPFLHAQRYVS GYGLQKGELSTLLYNTHPYR AFPVLLLDTVPWYLRLYVHT LTITSKGKENKPSYIHYQPA QDRLQPHLLEMLIQLPANSV TKVSIQFERALLKWTEYTPD P
Post Translational Modifications The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.
Function Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.
Protein Name Gpi Transamidase Component Pig-T
Phosphatidylinositol-Glycan Biosynthesis Class T Protein
Database Links Reactome: R-HSA-162791
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Gpi Transamidase Component Pig-T antibody
Anti-Phosphatidylinositol-Glycan Biosynthesis Class T Protein antibody
Anti-PIGT antibody
Anti-CGI-06 antibody
Anti-PSEC0163 antibody
Anti-UNQ716 antibody
Anti-PRO1379 antibody

Information sourced from Uniprot.org

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