• Western blot analysis of extracts of HeLa cells, using Phospho-Smad1 (Ser463/465) /Smad5 (Ser463/465) /Smad9 (Ser465/467) polyclonal antibody (STJ11100991) at 1:1000 dilution. HeLa cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-Smad1 (Ser463/465) /Smad5 (Ser463/465) /Smad9 (Ser465/467) polyclonal antibody (STJ11100991) at 1:1000 dilution. HeLa cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-Smad1/Smad5/Smad9-S463/465 & S463/465 & S465/467 antibody (STJ11100991)

SKU:
STJ11100991

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Smad1/Smad5/Smad9-S463/465 & S463/465 & S465/467 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD5
SMAD9
4090
4093
4086
SMAD5_HUMAN
SMAD9_HUMAN
SMAD1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S463 & S465 of human Smad1SMAD1 (NP_005891.1).
Immunogen Sequence: SSVS
Tissue Specificity Q99717 - Ubiquitous.
O15198 - Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
Q15797 - Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Q99717 - Ubiquitous.
O15198 - Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
Q15797 - Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 5 antibody
Anti-Mad Homolog 5 antibody
Anti-Mothers Against Dpp Homolog 5 antibody
Anti-Jv5-1 antibody
Anti-Smad Family Member 5 antibody
Anti-Smad 5 antibody
Anti-Smad5 antibody
Anti-Hsmad5 antibody
Anti-SMAD5 antibody
Anti-MADH5 antibody
Anti-Mothers Against Decapentaplegic Homolog 9 antibody
Anti-Mad Homolog 9 antibody
Anti-Mothers Against Dpp Homolog 9 antibody
Anti-Madh6 antibody
Anti-Smad Family Member 9 antibody
Anti-Smad 9 antibody
Anti-Smad9 antibody
Anti-SMAD9 antibody
Anti-MADH6 antibody
Anti-MADH9 antibody
Anti-SMAD8 antibody
Anti-Mothers Against Decapentaplegic Homolog 1 antibody
Anti-Mad Homolog 1 antibody
Anti-Mothers Against Dpp Homolog 1 antibody
Anti-Jv4-1 antibody
Anti-Mad-Related Protein 1 antibody
Anti-Smad Family Member 1 antibody
Anti-Smad 1 antibody
Anti-Smad1 antibody
Anti-Hsmad1 antibody
Anti-Transforming Growth Factor-Beta-Signaling Protein 1 antibody
Anti-Bsp-1 antibody
Anti-SMAD1 antibody
Anti-BSP1 antibody
Anti-MADH1 antibody
Anti-MADR1 antibody
Anti-Mothers Against Decapentaplegic Homolog 5 antibody
Anti-Mad Homolog 5 antibody
Anti-Mothers Against Dpp Homolog 5 antibody
Anti-Jv5-1 antibody
Anti-Smad Family Member 5 antibody
Anti-Smad 5 antibody
Anti-Smad5 antibody
Anti-Hsmad5 antibody
Anti-SMAD5 antibody
Anti-MADH5 antibody
Anti-Mothers Against Decapentaplegic Homolog 9 antibody
Anti-Mad Homolog 9 antibody
Anti-Mothers Against Dpp Homolog 9 antibody
Anti-Madh6 antibody
Anti-Smad Family Member 9 antibody
Anti-Smad 9 antibody
Anti-Smad9 antibody
Anti-SMAD9 antibody
Anti-MADH6 antibody
Anti-MADH9 antibody
Anti-SMAD8 antibody
Anti-Mothers Against Decapentaplegic Homolog 1 antibody
Anti-Mad Homolog 1 antibody
Anti-Mothers Against Dpp Homolog 1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance