• Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-spleen tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)
  • Immunofluorescence analysis of rat-spleen tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-stomach tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-stomach tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of human-lung tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of COS7 cells using Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody diluted at 1：1000
  • Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of human-lung tissue. 1, PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-PI3K p85/p55-Tyr467/199 antibody (436-485 aa) (STJ90384)

SKU:
STJ90384

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Phosphatidylinositol 3-kinase regulatory subunit alpha and Phosphatidylinositol 3-kinase regulatory subunit gamma-Tyr467/199 (436-485 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluores
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIK3R1
PIK3R3
Gene ID: 5295
8503
Uniprot ID: P85A_HUMAN
P55G_HUMAN
Immunogen Region: 436-485 aa
Specificity: Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85/p55 protein only when phosphorylated at Y467/199.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199 at the amino acid range 436-485
Post Translational Modifications Polyubiquitinated in T-cells by CBLB.which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608.phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
Function Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Protein Name Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha
Pi3-Kinase Regulatory Subunit Alpha
Pi3k Regulatory Subunit Alpha
Ptdins-3-Kinase Regulatory Subunit Alpha
Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha
Pi3-Kinase Subunit P85-Alpha
Ptdins-3-Kinase Regulatory Subunit P85-Alpha
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-112399
Reactome: R-HSA-114604
Reactome: R-HSA-1236382
Reactome: R-HSA-1250342
Reactome: R-HSA-1257604
Reactome: R-HSA-1266695
Reactome: R-HSA-1433557
Reactome: R-HSA-1660499
Reactome: R-HSA-180292
Reactome: R-HSA-1839117
Reactome: R-HSA-186763
Reactome: R-HSA-1963642
Reactome: R-HSA-198203
Reactome: R-HSA-202424
Reactome: R-HSA-2029485
Reactome: R-HSA-210993
Reactome: R-HSA-2219530
Reactome: R-HSA-2424491
Reactome: R-HSA-2730905
Reactome: R-HSA-373753
Reactome: R-HSA-388841
Reactome: R-HSA-389357
Reactome: R-HSA-416476
Reactome: R-HSA-430116
Reactome: R-HSA-4420097
Reactome: R-HSA-512988
Reactome: R-HSA-5637810
Reactome: R-HSA-5654689
Reactome: R-HSA-5654695
Reactome: R-HSA-5654710
Reactome: R-HSA-5654720
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-5673001
Reactome: R-HSA-6785807
Reactome: R-HSA-6811558
Reactome: R-HSA-8851907
Reactome: R-HSA-8853334
Reactome: R-HSA-8853338
Reactome: R-HSA-8853659
Reactome: R-HSA-9009391
Reactome: R-HSA-9027276
Reactome: R-HSA-9028335
Reactome: R-HSA-912526
Reactome: R-HSA-912631
Reactome: R-HSA-9603381
Reactome: R-HSA-9607240
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9670439
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-983695
Alternative Antibody Names Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha antibody
Anti-Pi3-Kinase Regulatory Subunit Alpha antibody
Anti-Pi3k Regulatory Subunit Alpha antibody
Anti-Ptdins-3-Kinase Regulatory Subunit Alpha antibody
Anti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha antibody
Anti-Pi3-Kinase Subunit P85-Alpha antibody
Anti-Ptdins-3-Kinase Regulatory Subunit P85-Alpha antibody
Anti-PIK3R1 antibody
Anti-GRB1 antibody

Information sourced from Uniprot.org

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