• Western blot analysis of extracts of C2C12 cells, using Phospho-AMPK Beta 1-S182 antibody (STJ11103734) at 1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Phospho-AMPK Beta 1-S182 in paraffin-embedded mouse testis using Phospho-AMPK Beta 1-S182 Rabbit polyclonal antibody (STJ11103734) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using Phospho-AMPK Beta 1-S182 rabbit polyclonal antibody (STJ11103734) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from C2C12 cells, using Phospho-AMPK Beta 1-S182 Rabbit polyclonal antibody (STJ11103734) at 1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-AMPK Beta 1-S182 antibody (STJ11103734)

SKU:
STJ11103734

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-AMPK Beta 1-S182 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAB1
Gene ID: 5564
Uniprot ID: AAKB1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S182 of human AMPK Beta 1 (NP_006244.2).
Immunogen Sequence: SSSPP
Post Translational Modifications Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Protein Name 5'-Amp-Activated Protein Kinase Subunit Beta-1
Ampk Subunit Beta-1
Ampkb
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1632852
Reactome: R-HSA-2151209
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-6804756
Reactome: R-HSA-9613354
Reactome: R-HSA-9619483
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Subunit Beta-1 antibody
Anti-Ampk Subunit Beta-1 antibody
Anti-Ampkb antibody
Anti-PRKAB1 antibody
Anti-AMPK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance