• Immunohistochemistry analysis of paraffin-embedded human appendix using PGAM1 Rabbit monoclonal antibody (STJ11102009) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PGAM1 Rabbit monoclonal antibody (STJ11102009) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PGAM1 antibody (1-100) [S9MR] (STJ11102009)

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STJ11102009

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PGAM1 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGAM1
Gene ID: 5223
Uniprot ID: PGAM1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PGAM1 (P18669).
Immunogen Sequence: MAAYKLVLIRHGESAWNLEN RFSGWYDADLSPAGHEEAKR GGQALRDAGYEFDICFTSVQ KRAIRTLWTVLDAIDQMWLP VVRTWRLNERHYGGLTGLNK
Tissue Specificity Expressed in the liver and brain. Not found in the muscle.
Post Translational Modifications Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme.
Function Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglyceratea crucial step in glycolysis, by using 2,3-bisphosphoglycerate. Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate.
Protein Name Phosphoglycerate Mutase 1
Bpg-Dependent Pgam 1
Phosphoglycerate Mutase Isozyme B
Pgam-B
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Reactome: R-HSA-70263
Alternative Antibody Names Anti-Phosphoglycerate Mutase 1 antibody
Anti-Bpg-Dependent Pgam 1 antibody
Anti-Phosphoglycerate Mutase Isozyme B antibody
Anti-Pgam-B antibody
Anti-PGAM1 antibody
Anti-PGAMA antibody
Anti-CDABP0006 antibody

Information sourced from Uniprot.org

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