• Western blot analysis of extracts of HepG2 cells, using PDZK1 antibody (STJ11103637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of mouse kidney using PDZK1 Rabbit monoclonal antibody (STJ11103637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat kidney using PDZK1 rabbit monoclonal antibody (STJ11103637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat kidney using PDZK1 Rabbit monoclonal antibody (STJ11103637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse kidney using PDZK1 rabbit monoclonal antibody (STJ11103637) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PDZK1 antibody (420-519) [S7MR] (STJ11103637)

SKU:
STJ11103637

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Host: Rabbit
Applications: IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PDZK1 (420-519) is suitable for use in Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDZK1
Gene ID: 5174
Uniprot ID: NHRF3_HUMAN
Immunogen Region: 420-519
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 420-519 of human PDZK1 (Q5T2W1).
Immunogen Sequence: EDVIIEVNGVNVLDEPYEKV VDRIQSSGKNVTLLVCGKKA YDYFQAKKIPIVSSLADPLD TPPDSKEGIVVESNHDSHMA KERAHSTASHSSSNSEDTEM
Tissue Specificity Expression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas.
Function A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with NHERF1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
Protein Name Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf3
Nherf-3
Cftr-Associated Protein Of 70 Kda
Na(+/H(+ Exchanger Regulatory Factor 3
Na/Pi Cotransporter C-Terminal-Associated Protein 1
Napi-Cap1
Pdz Domain-Containing Protein 1
Sodium-Hydrogen Exchanger Regulatory Factor 3
Cellular Localisation Membrane
Peripheral Membrane Protein
Cell Membrane
Associated With Peripheral Membranes
Localizes To The Apical Compartment Of Proximal Tubular Cells And To Sinusoidal Liver Membranes
Alternative Antibody Names Anti-Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf3 antibody
Anti-Nherf-3 antibody
Anti-Cftr-Associated Protein Of 70 Kda antibody
Anti-Na(+/H(+ Exchanger Regulatory Factor 3 antibody
Anti-Na/Pi Cotransporter C-Terminal-Associated Protein 1 antibody
Anti-Napi-Cap1 antibody
Anti-Pdz Domain-Containing Protein 1 antibody
Anti-Sodium-Hydrogen Exchanger Regulatory Factor 3 antibody
Anti-PDZK1 antibody
Anti-CAP70 antibody
Anti-NHERF3 antibody
Anti-PDZD1 antibody

Information sourced from Uniprot.org

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