| Host: |
Rabbit |
| Applications: |
IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PDHA1 (30-390) is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC 1:50-1:200IF 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PDHA1 |
| Gene ID: |
5160 |
| Uniprot ID: |
ODPA_HUMAN |
| Immunogen Region: |
30-390 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). |
| Immunogen Sequence: |
FANDATFEIKKCDLHRLEEG PPVTTVLTREDGLKYYRMMQ TVRRMELKADQLYKQKIIRG FCHLCDGQEACCVGLEAGIN PTDHLITAYRAHGFTFTRGL SVREILAELTGRKGGCAKGK GGSMHMYAKNFYGGNGIVGA QVPLGAGIALACKYNGKDEV CLTLYGDGAANQGQIFEAYN MAALWKLPCIFICENNRYGM GTSVERAAASTDYYKRGDFI PGLRVDGMDILCVREATRF |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme.for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3. |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Protein Name | Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - MitochondrialPdhe1-A Type I |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-389661Reactome: R-HSA-5362517Reactome: R-HSA-70268 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Pyruvate Dehydrogenase E1 Component Subunit Alpha - Somatic Form - Mitochondrial antibodyAnti-Pdhe1-A Type I antibodyAnti-PDHA1 antibodyAnti-PHE1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
