| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S7MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDCD4 |
| Gene ID: | 27250 |
| Uniprot ID: | PDCD4_HUMAN |
| Immunogen Region: | 370-469 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 370-469 of human PDCD4 (Q53EL6). |
| Immunogen Sequence: | MVLESTGESTFKMILDLLKS LWKSSTITVDQMKRGYERIY NEIPDINLDVPHSYSVLERF VEECFQAGIISKQLRDLCPS RGRKRFVSEGDGGRLKPESY |
| Tissue Specificity | Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. |
| Post Translational Modifications | Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens.phosphorylation promotes proteasomal degradation of PDCD4. |
| Function | Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. |
| Protein Name | Programmed Cell Death Protein 4 Neoplastic Transformation Inhibitor Protein Nuclear Antigen H731-Like Protein 197/15a |
| Database Links | Reactome: R-HSA-8950505 |
| Cellular Localisation | Nucleus Cytoplasm Shuttles Between The Nucleus And Cytoplasm Predominantly Nuclear Under Normal Growth Conditions And When Phosphorylated At Ser-457 |
| Alternative Antibody Names | Anti-Programmed Cell Death Protein 4 antibody Anti-Neoplastic Transformation Inhibitor Protein antibody Anti-Nuclear Antigen H731-Like antibody Anti-Protein 197/15a antibody Anti-PDCD4 antibody Anti-H731 antibody |
Information sourced from Uniprot.org