• Western blot analysis of extracts of Rat kidney, using PCGF2 antibody (STJ119456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PCGF2 antibody (115-241) (STJ119456)

SKU:
STJ119456

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCGF2 (115-241) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCGF2
Gene ID: 7703
Uniprot ID: PCGF2_HUMAN
Immunogen Region: 115-241
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 115-241 of human PCGF2 (NP_009075.1).
Immunogen Sequence: GEVLEQEKGALSDDEIVSLS IEFYEGARDRDEKKGPLENG DGDKEKTGVRFLRCPAAMTV MHLAKFLRNKMDVPSKYKVE VLYEDEPLKEYYTLMDIAYI YPWRRNGPLPLKYRVQPACK RLTLATV
Tissue Specificity Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.
Post Translational Modifications Phosphorylated. Homodimer formation is regulated by phosphorylation with only unphosphorylated proteins forming homodimers.
Function Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.
Protein Name Polycomb Group Ring Finger Protein 2
Dna-Binding Protein Mel-18
Ring Finger Protein 110
Zinc Finger Protein 144
Database Links Reactome: R-HSA-3108214
Reactome: R-HSA-3899300
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4655427
Reactome: R-HSA-5617472
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Polycomb Group Ring Finger Protein 2 antibody
Anti-Dna-Binding Protein Mel-18 antibody
Anti-Ring Finger Protein 110 antibody
Anti-Zinc Finger Protein 144 antibody
Anti-PCGF2 antibody
Anti-MEL18 antibody
Anti-RNF110 antibody
Anti-ZNF144 antibody

Information sourced from Uniprot.org

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