• Western blot analysis of lysates from Mouse liver using PCCB Rabbit polyclonal antibody (STJ11104391) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PCCB antibody (260-539) (STJ11104391)

SKU:
STJ11104391

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Propionyl-Coa Carboxylase Beta Chain-Mitochondrial (260-539) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCCB
Gene ID: 5096
Uniprot ID: PCCB_HUMAN
Immunogen Region: 260-539
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 260-539 of human PCCB (NP_000523.2).
Immunogen Sequence: AFENDVDALCNLRDFFNYLP LSSQDPAPVRECHDPSDRLV PELDTIVPLESTKAYNMVDI IHSVVDEREFFEIMPNYAKN IIVGFARMNGRTVGIVGNQP KVASGCLDINSSVKGARFVR FCDAFNIPLITFVDVPGFLP GTAQEYGGIIRHGAKLLYAF AEATVPKVTVITRKAYGGAY DVMSSKHLCGDTNYAWPTAE IAVMGAKGAVEIIFKGHENV EAAQAEYIEKFANPFPAAV
Function This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA.
Protein Name Propionyl-Coa Carboxylase Beta Chain - Mitochondrial
Pccase Subunit Beta
Propanoyl-Coa -Carbon Dioxide Ligase Subunit Beta
Database Links Reactome: R-HSA-196780
Reactome: R-HSA-3371599
Reactome: R-HSA-71032
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Propionyl-Coa Carboxylase Beta Chain - Mitochondrial antibody
Anti-Pccase Subunit Beta antibody
Anti-Propanoyl-Coa -Carbon Dioxide Ligase Subunit Beta antibody
Anti-PCCB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance