• Western blot analysis of extracts of various cell lines, using PABPN1 rabbit monoclonal antibody (STJ11101447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded human cervix cancer tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded human liver cancer tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded human lung squamous carcinoma tissue tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded human thyroid cancer tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded human tonsil tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded mouse intestin tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded mouse liver tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded mouse spleen tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded mouse testis tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded rat brain tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded rat colon tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded rat kidney tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded rat liver tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded rat lung tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PABPN1 in paraffin-embedded rat spleen tissue using PABPN1 rabbit monoclonal antibody (STJ11101447) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using PABPN1 rabbit monoclonal antibody (STJ11101447) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PABPN1 rabbit monoclonal antibody (STJ11101447) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using PABPN1 rabbit monoclonal antibody (STJ11101447) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PABPN1 antibody (1-100) [S7MR] (STJ11101447)

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STJ11101447

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PABPN1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PABPN1
Gene ID: 8106
Uniprot ID: PABP2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (Q86U42).
Immunogen Sequence: MAAAAAAAAAAGAAGGRGSG PGRRRHLVPGAGGEAGEGAP GGAGDYGNGLESEELEPEEL LLEPEPEPEPEEEPPRPRAP PGAPGPGPGSGAPGSQEEEE
Tissue Specificity Ubiquitous.
Post Translational Modifications Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties.
Function Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters.
Protein Name Polyadenylate-Binding Protein 2
Pabp-2
Poly(A-Binding Protein 2
Nuclear Poly(A-Binding Protein 1
Poly(A-Binding Protein Ii
Pabii
Polyadenylate-Binding Nuclear Protein 1
Database Links Reactome: R-HSA-168315
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Cytoplasm
Nucleus Speckle
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Shuttles Between The Nucleus And The Cytoplasm But Predominantly Found In The Nucleus
Its Nuclear Import May Involve The Nucleocytoplasmic Transport Receptor Transportin And A Ran-Gtp-Sensitive Import Mechanism
Is Exported To The Cytoplasm By A Carrier-Mediated Pathway That Is Independent Of Mrna Traffic
Colocalizes With Skip And Poly(A) Rna In Nuclear Speckles
Intranuclear Filamentous Inclusions Or 'Aggregates' Are Detected In The Myocytes Of Patients
These Inclusions Contain Pabpn1
Ubiquitin
Subunits Of The Proteasome And Poly(A) Rna
Alternative Antibody Names Anti-Polyadenylate-Binding Protein 2 antibody
Anti-Pabp-2 antibody
Anti-Poly(A-Binding Protein 2 antibody
Anti-Nuclear Poly(A-Binding Protein 1 antibody
Anti-Poly(A-Binding Protein Ii antibody
Anti-Pabii antibody
Anti-Polyadenylate-Binding Nuclear Protein 1 antibody
Anti-PABPN1 antibody
Anti-PAB2 antibody
Anti-PABP2 antibody

Information sourced from Uniprot.org

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