• Immunohistochemistry analysis of paraffin-embedded human esophageal using JunB Rabbit monoclonal antibody (STJ11102167) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of BxPC-3 cells, using JunB Rabbit monoclonal antibody (STJ11102167) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-JUNB antibody (1-100) [S7MR] (STJ11102167)

SKU:
STJ11102167

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-JUNB (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JUNB
Gene ID: 3726
Uniprot ID: JUNB_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JunB (P17275).
Immunogen Sequence: MCTKMEQPFYHDDSYTATGY GRAPGGLSLHDYKLLKPSLA VNLADPYRSLKAPGARGPGP EGGGGGSYFSGQGSDTGASL KLASSELERLIVPNSNGVIT
Post Translational Modifications Ubiquitinated by ITCH, leading to its degradation.
Function Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGAGCTCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity.
Protein Name Transcription Factor Junb
Transcription Factor Ap-1 Subunit Junb
Database Links Reactome: R-HSA-2173796
Reactome: R-HSA-6785807
Reactome: R-HSA-9031628
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Junb antibody
Anti-Transcription Factor Ap-1 Subunit Junb antibody
Anti-JUNB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance