• STJ70458 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70458 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-ORP3 antibody (N-Term) (STJ70458)

SKU:
STJ70458-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ORP3 (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of A431 cells. 10µg/ml
FC-Flow cytometric analysis of A431 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: OSBPL3
Gene ID: 26031
Uniprot ID: OSBL3_HUMAN
Immunogen Region: N-Term
Accession Number: NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1
Specificity: This antibody is expected to recognise all reported isoforms (NP_056365.1; NP_663160.1; NP_663161.1; NP_663162.1).
Immunogen Sequence: MSDEKNLGVSQKL
Post Translational Modifications Phosphorylation is enhanced in vitro by phorbol-12-myristate-13-acetate (PMA), forskolin and calcium ionophore A23187. Phosphorylation seems to be stimulated in conditions of low cell-cell (or cell-matrix) adhesion.
Function Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and cholesterol.
Protein Name Oxysterol-Binding Protein-Related Protein 3
Orp-3
Osbp-Related Protein 3
Database Links Reactome: R-HSA-192105
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
Cell Membrane
Cell Projection
Filopodium Tip
Nucleus Membrane
Co-Localizes With Osbpl6 At Contact Sites Between The Plasma Membrane And The Endoplasmic Reticulum
Alternative Antibody Names Anti-Oxysterol-Binding Protein-Related Protein 3 antibody
Anti-Orp-3 antibody
Anti-Osbp-Related Protein 3 antibody
Anti-OSBPL3 antibody
Anti-KIAA0704 antibody
Anti-ORP3 antibody
Anti-OSBP3 antibody

Information sourced from Uniprot.org

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