• Western blot analysis of extracts of various cell lines, using NUMB antibody (STJ11103644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g NUMB antibody (STJ11103644). Western blot was performed from the immunoprecipitate using NUMB (STJ11103644) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug NUMB antibody (STJ11103644). Western blot was performed from the immunoprecipitate using NUMB (STJ11103644) at a dilition of 1:1000.
  • Western blot analysis of various lysates using NUMB Rabbit monoclonal antibody (STJ11103644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NUMB antibody (1-100) [S4MR] (STJ11103644)

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STJ11103644

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NUMB (1-100) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUMB
Gene ID: 8650
Uniprot ID: NUMB_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUMB (P49757).
Immunogen Sequence: MNKLRQSFRRKKDVYVPEAS RPHQWQTDEEGVRTGKCSFP VKYLGHVEVDESRGMHICED AVKRLKAERKFFKGFFGKTG KKAVKAVLWVSADGLRVVDE
Post Translational Modifications Phosphorylated on Ser-276 and Ser-295 by CaMK1. Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation. Ubiquitinated.mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Function Regulates clathrin-mediated receptor endocytosis. Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Protein Name Protein Numb Homolog
H-Numb
Protein S171
Database Links Reactome: R-HSA-2122948
Reactome: R-HSA-437239
Reactome: R-HSA-5610780
Reactome: R-HSA-5632684
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endosome Membrane
Localizes To Perinuclear Endosomes In An Aak1-Dependent Manner
Alternative Antibody Names Anti-Protein Numb Homolog antibody
Anti-H-Numb antibody
Anti-Protein S171 antibody
Anti-NUMB antibody
Anti-C14orf41 antibody

Information sourced from Uniprot.org

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