• Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g NSF antibody (STJ29825). Western blot was performed from the immunoprecipitate using NSF antibody (STJ29825) at a dilution of 1:3000.
  • Immunofluorescence analysis of U-2 OS cells using NSF Rabbit polyclonal antibody (STJ29825) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of BALB-3T3 cells using NSF Rabbit polyclonal antibody (STJ29825) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NSF Rabbit polyclonal antibody (STJ29825) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NSF antibody (251-400) (STJ29825)

SKU:
STJ29825

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSF
Gene ID: 4905
Uniprot ID: NSF_HUMAN
Immunogen Region: 251-400
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 251-400 of human NSF (NP_006169.2).
Immunogen Sequence: KHVKGILLYGPPGCGKTLLA RQIGKMLNAREPKVVNGPEI LNKYVGESEANIRKLFADAE EEQRRLGANSGLHIIIFDEI DAICKQRGSMAGSTGVHDTV VNQLLSKIDGVEQLNNILVI GMTNRPDLIDEALLRPGRLE VKMEIGLPDE
Post Translational Modifications Phosphorylation at Ser-569 interferes with homohexamerization.
Function Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
Protein Name Vesicle-Fusing Atpase
N-Ethylmaleimide-Sensitive Fusion Protein
Nem-Sensitive Fusion Protein
Vesicular-Fusion Protein Nsf
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-416993
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811438
Reactome: R-HSA-6811440
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Vesicle-Fusing Atpase antibody
Anti-N-Ethylmaleimide-Sensitive Fusion Protein antibody
Anti-Nem-Sensitive Fusion Protein antibody
Anti-Vesicular-Fusion Protein Nsf antibody
Anti-NSF antibody

Information sourced from Uniprot.org

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