| Host: | Rabbit |
| Applications: | WB/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-NRG1 (1-210) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NRG1 |
| Gene ID: | 3084 |
| Uniprot ID: | NRG1_HUMAN |
| Immunogen Region: | 1-210 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human NRG1 (NP_039252.2). |
| Immunogen Sequence: | MSERKEGRGKGKGKKKERGS GKKPESAAGSQSPALPPRLK EMKSQESAAGSKLVLRCETS SEYSSLRFKWFKNGNELNRK NKPQNIKIQKKPGKSELRIN KASLADSGEYMCKVISKLGN DSASANITIVESNEIITGMP ASTEGAYVSSESPIRISVST EGANTSSSTSTSTTGTSHLV KCAEKEKTFCVNGGECFMVK DLSNPSRYLC |
| Tissue Specificity | Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells.in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. |
| Post Translational Modifications | Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage. |
| Function | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells.inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction.stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells.stimulating Schwann cell proliferation.implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons. |
| Protein Name | Pro-Neuregulin-1 - Membrane-Bound IsoformPro-Nrg1 Cleaved Into - Neuregulin-1Acetylcholine Receptor-Inducing ActivityAriaBreast Cancer Cell Differentiation Factor P45Glial Growth FactorHeregulinHrgNeu Differentiation FactorSensory And Motor Neuron-Derived Factor |
| Database Links | Reactome: R-HSA-1227986 Q02297-10Reactome: R-HSA-1236394 Q02297-10Reactome: R-HSA-1250196 Q02297-10Reactome: R-HSA-1250342Reactome: R-HSA-1250347Reactome: R-HSA-1251985Reactome: R-HSA-1257604 Q02297-10Reactome: R-HSA-1306955 Q02297-10Reactome: R-HSA-1358803 Q02297-10Reactome: R-HSA-1963640Reactome: R-HSA-1963642 Q02297-10Reactome: R-HSA-2219530 Q02297-10Reactome: R-HSA-5673001 Q02297-10Reactome: R-HSA-6785631 Q02297-10Reactome: R-HSA-6811558 Q02297-10Reactome: R-HSA-8847993 Q02297-10Reactome: R-HSA-8863795 Q02297-10Reactome: R-HSA-9620244Reactome: R-HSA-9664565 Q02297-10Reactome: R-HSA-9665686 Q02297-10 |
| Cellular Localisation | Pro-Neuregulin-1Membrane-Bound Isoform: Cell MembraneSingle-Pass Type I Membrane ProteinDoes Not Seem To Be ActiveNeuregulin-1: SecretedIsoform 8: NucleusMay Be NuclearIsoform 9: SecretedHas A Signal PeptideIsoform 10: MembraneMay Possess An Internal Uncleaved Signal Sequence |
| Alternative Antibody Names | Anti-Pro-Neuregulin-1 - Membrane-Bound Isoform antibodyAnti-Pro-Nrg1 Cleaved Into - Neuregulin-1 antibodyAnti-Acetylcholine Receptor-Inducing Activity antibodyAnti-Aria antibodyAnti-Breast Cancer Cell Differentiation Factor P45 antibodyAnti-Glial Growth Factor antibodyAnti-Heregulin antibodyAnti-Hrg antibodyAnti-Neu Differentiation Factor antibodyAnti-Sensory And Motor Neuron-Derived Factor antibodyAnti-NRG1 antibodyAnti-GGF antibodyAnti-HGL antibodyAnti-HRGA antibodyAnti-NDF antibodyAnti-SMDF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
