• Immunofluorescence analysis of A-549 cells using NMT2 antibody (STJ29122). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using NMT2 antibody (STJ29122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NMT2 antibody (1-120) (STJ29122)

SKU:
STJ29122

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NMT2 (1-120) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NMT2
Gene ID: 9397
Uniprot ID: NMT2_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NMT2 (NP_004799.1).
Immunogen Sequence: MAEDSESAASQQSLELDDQD TCGIDGDNEEETEHAKGSPG GYLGAKKKKKKQKRKKEKPN SGGTKSDSASDSQEIKIQQP SKNPSVPMQKLQDIQRAMEL LSACQGPARNIDEAAKHRYQ
Function Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Also able to mediate N-terminal lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3'. Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle.
Protein Name Glycylpeptide N-Tetradecanoyltransferase 2
Myristoyl-Coa -Protein N-Myristoyltransferase 2
Nmt 2
Peptide N-Myristoyltransferase 2
Protein-Lysine Myristoyltransferase Nmt2
Type Ii N-Myristoyltransferase
Database Links Reactome: R-HSA-174490
Reactome: R-HSA-2514859
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Glycylpeptide N-Tetradecanoyltransferase 2 antibody
Anti-Myristoyl-Coa -Protein N-Myristoyltransferase 2 antibody
Anti-Nmt 2 antibody
Anti-Peptide N-Myristoyltransferase 2 antibody
Anti-Protein-Lysine Myristoyltransferase Nmt2 antibody
Anti-Type Ii N-Myristoyltransferase antibody
Anti-NMT2 antibody

Information sourced from Uniprot.org

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