• Western blot analysis of extracts of various cell lines, using NLRC4 antibody (STJ115083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
  • Immunohistochemistry of paraffin-embedded human lung using NLRC4 antibody (STJ115083) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using NLRC4 antibody (STJ115083) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using NLRC4 antibody (STJ115083) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NLRC4 antibody (89-159) (STJ115083)

SKU:
STJ115083

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NLRC4 (89-159) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NLRC4
Gene ID: 58484
Uniprot ID: NLRC4_HUMAN
Immunogen Region: 89-159
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 89-159 of human NLRC4 (NP_001028539.1).
Immunogen Sequence: LSYQVTEEDLNVLAQNLKDL YNSPAFLNFYPLGEDIDIIF NLEKTFTEPIMWKKDHRHHR VEQLTLGSLLE
Tissue Specificity Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
Post Translational Modifications Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro.
Function Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria.
Protein Name Nlr Family Card Domain-Containing Protein 4
Card - Lrr - And Nacht-Containing Protein
Ced-4-Like Protein Clan
Caspase Recruitment Domain-Containing Protein 12
Ice Protease-Activating Factor
Ipaf
Database Links Reactome: R-HSA-6803207
Reactome: R-HSA-844623
Cellular Localisation Cytoplasm
Cytosol
Inflammasome
Alternative Antibody Names Anti-Nlr Family Card Domain-Containing Protein 4 antibody
Anti-Card - Lrr - And Nacht-Containing Protein antibody
Anti-Ced-4-Like Protein Clan antibody
Anti-Caspase Recruitment Domain-Containing Protein 12 antibody
Anti-Ice Protease-Activating Factor antibody
Anti-Ipaf antibody
Anti-NLRC4 antibody
Anti-CARD12 antibody
Anti-CLAN antibody
Anti-CLAN1 antibody
Anti-IPAF antibody
Anti-UNQ6189 antibody
Anti-PRO20215 antibody

Information sourced from Uniprot.org

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