| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NIN (380-665) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NIN |
| Gene ID: |
51199 |
| Uniprot ID: |
NIN_HUMAN |
| Immunogen Region: |
380-665 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 380-665 of human NIN (NP_891991.1). |
| Immunogen Sequence: |
VREKEKLRSDLDKAEKLKSL MASEVDDHHAAIERRNEYNL RKLDEEYKERIAALKNELRK EREQILQQAGKQRLELEQEI EKAKTEENYIRDRLALSLKE NSRLENELLENAEKLAEYEN LTNKLQRNLENVLAEKFGDL DPSSAEFFLQEERLTQMRNE YERQCRVLQDQVDELQSELE EYRAQGRVLRLPLKNSPSEE VEANSGGIEPEHGLGSEECN PLNMSIEAELVIEQMKEQH |
| Tissue Specificity | Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. |
| Post Translational Modifications | Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B. |
| Function | Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Required for centriole organization and microtubule anchoring at the mother centriole. |
| Protein Name | NineinHnineinGlycogen Synthase Kinase 3 Beta-Interacting ProteinGsk3b-Interacting Protein |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleComponent Of The Core CentrosomeArranged In A Tubular Conformation With An Open And A Closed End Within The CentrosomeIn The Mother CentrosomeIt Localizes At Both Ends Of The Centrosome TubeIncluding The Site Of Centrosome DuplicationWhile In The Daughter Centrosome It Is Present Only At The Closed EndRequires Pcm1 For Centrosome LocalizationLocalizes To The Subdistal Appendage Region Of The Centriole In A Dctn1-Dependent MannerIsoform 6: CytoplasmSeems To Have A Dominant-Negative Effect On Localization Of Other IsoformsPromoting Their Dissociation From The Centrosome |
| Alternative Antibody Names | Anti-Ninein antibodyAnti-Hninein antibodyAnti-Glycogen Synthase Kinase 3 Beta-Interacting Protein antibodyAnti-Gsk3b-Interacting Protein antibodyAnti-NIN antibodyAnti-KIAA1565 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
