| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NELFB (349-628) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NELFB |
| Gene ID: |
25920 |
| Uniprot ID: |
NELFB_HUMAN |
| Immunogen Region: |
349-628 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 349-628 of human NELFB (NP_056271.3). |
| Immunogen Sequence: |
LSMILCDPFAINTLALSTVR HLQELVGQETLPRDSPDLLL LLRLLALGQGAWDMIDSQVF KEPKMEVELITRFLPMLMSF LVDDYTFNVDQKLPAEEKAP VSYPNTLPESFTKFLQEQRM ACEVGLYYVLHITKQRNKNA LLRLLPGLVETFGDLAFGDI FLHLLTGNLALLADEFALED FCSSLFDGFFLTASPRKENV HRHALRLLIHLHPRVAPSKL EALQKALEPTGQSGEAVKE |
| Tissue Specificity | Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. |
| Function | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Essential for early embryogenesis.plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes. Plays a key role in establishing the responsiveness of stem cells to developmental cues.facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules. Supports the transcription of genes involved in energy metabolism in cardiomyocytes.facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. |
| Protein Name | Negative Elongation Factor BNelf-BCofactor Of Brca1 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-75955Reactome: R-HSA-9603505 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Negative Elongation Factor B antibodyAnti-Nelf-B antibodyAnti-Cofactor Of Brca1 antibodyAnti-NELFB antibodyAnti-COBRA1 antibodyAnti-KIAA1182 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
