| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NEK4 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NEK4 |
| Gene ID: |
6787 |
| Uniprot ID: |
NEK4_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK4 (NP_003148.2). |
| Immunogen Sequence: |
MPLAAYCYLRVVGKGSYGEV TLVKHRRDGKQYVIKKLNLR NASSRERRAAEQEAQLLSQL KHPNIVTYKESWEGGDGLLY IVMGFCEGGDLYRKLKEQKG QLLPENQVVEWFVQIAMALQ YLHEKHILHRDLKTQNVFLT RTNIIKVGDLGIARVLENHC DMASTLIGTPYYMSPELFSN KPYNYKSDVWALGCCVYEMA TLKHAFNAKDMNSLVYRIIE GKLPPMPRDYSPELAELIR |
| Tissue Specificity | Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, testis, retina, liver, kidney, lung and placenta. Present in most primary carcinomas. |
| Function | Protein kinase that seems to act exclusively upon threonine residues. Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage. |
| Protein Name | Serine/Threonine-Protein Kinase Nek4Never In Mitosis A-Related Kinase 4Nima-Related Protein Kinase 4Serine/Threonine-Protein Kinase 2Serine/Threonine-Protein Kinase Nrk2 |
| Cellular Localisation | Cell ProjectionCiliumCytoplasm |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek4 antibodyAnti-Never In Mitosis A-Related Kinase 4 antibodyAnti-Nima-Related Protein Kinase 4 antibodyAnti-Serine/Threonine-Protein Kinase 2 antibodyAnti-Serine/Threonine-Protein Kinase Nrk2 antibodyAnti-NEK4 antibodyAnti-STK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
