• Immunoprecipitation analysis of 600 Mu g extracts of Rat brain cells using 3 Mu g Neurofilament H antibody (STJ11101621). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (STJ11101621) at a dilution of 1:500.
  • Confocal imaging of frozen sections of mouse brain using Neurofilament H Rabbit monoclonal antibody (STJ11101621, dilution 1:100) followed by incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Neurofilament H in paraffin-embedded rat brain tissue using Neurofilament H Rabbit monoclonal antibody (STJ11101621) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Neurofilament H in paraffin-embedded mouse brain tissue using Neurofilament H Rabbit monoclonal antibody (STJ11101621) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Neurofilament H in paraffin-embedded human brain tissue using Neurofilament H Rabbit monoclonal antibody (STJ11101621) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Neurofilament H Rabbit monoclonal antibody (STJ11101621) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NEFH antibody (550-650) [S1MR] (STJ11101621)

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STJ11101621

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NEFH (550-650) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFH
Gene ID: 4744
Uniprot ID: NFH_HUMAN
Immunogen Region: 550-650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human Neurofilament H (P12036).
Immunogen Sequence: AKSPAKEEAKSPPEAKSPEK EEAKSPAEVKSPEKAKSPAK EEAKSPAEAKSPEKAKSPVK EEAKSPAEAKSPVKEEAKSP AEVKSPEKAKSPTKEEAKSP E
Post Translational Modifications There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Heavy Polypeptide
Nf-H
200 Kda Neurofilament Protein
Neurofilament Triplet H Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Axon
Alternative Antibody Names Anti-Neurofilament Heavy Polypeptide antibody
Anti-Nf-H antibody
Anti-200 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet H Protein antibody
Anti-NEFH antibody
Anti-KIAA0845 antibody
Anti-NFH antibody

Information sourced from Uniprot.org

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