• Western blot analysis of lysates from wild type (WT) and NDUFV2 knockout (KO) HeLa cells, using [KO Validated] NDUFV2 Rabbit polyclonal antibody (STJ11100812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NDUFV2 antibody (1-249) (STJ11100812)

SKU:
STJ11100812

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFV2 (1-249) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFV2
Gene ID: 4729
Uniprot ID: NDUV2_HUMAN
Immunogen Region: 1-249
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human NDUFV2 (NP_066552.2).
Immunogen Sequence: MFFSAALRARAAGLTAHWGR HVRNLHKTVMQNGAGGALFV HRDTPENNPDTPFDFTPENY KRIEAIVKNYPEGHKAAAVL PVLDLAQRQNGWLPISAMNK VAEVLQVPPMRVYEVATFYT MYNRKPVGKYHIQVCTTTPC MLRNSDSILEAIQKKLGIKV GETTPDKLFTLIEVECLGAC VNAPMVQINDNYYEDLTAKD IEEIIDELKAGKIPKPGPRS GRFSCEPAGGLTSLTEPPK
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.
Protein Name Nadh Dehydrogenase Ubiquinone Flavoprotein 2 - Mitochondrial
Nadh-Ubiquinone Oxidoreductase 24 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Flavoprotein 2 - Mitochondrial antibody
Anti-Nadh-Ubiquinone Oxidoreductase 24 Kda Subunit antibody
Anti-NDUFV2 antibody

Information sourced from Uniprot.org

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