• Western blot analysis of extracts of HeLa cells, using MYO1E antibody (STJ114999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MYO1E antibody (809-1108) (STJ114999)

SKU:
STJ114999

€211.88 - €561.88
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYO1E (809-1108) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYO1E
Gene ID: 4643
Uniprot ID: MYO1E_HUMAN
Immunogen Region: 809-1108
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 809-1108 of human MYO1E (NP_004989.2).
Immunogen Sequence: KEVLKRKIEIERILSVSLST MQDDIFILHEQEYDSLLESV FKTEFLSLLAKRYEEKTQKQ LPLKFSNTLELKLKKENWGP WSAGGSRQVQFHQGFGDLAV LKPSNKVLQVSIGPGLPKNS RPTRRNTTQNTGYSSGTQNA NYPVRAAPPPPGYHQNGVIR NQYVPYPHAPGSQRSNQKSL YTSMARPPLPRQQSTSSDRV SQTPESLDFLKVPDQGAAGV RRQTTSRPPPAGGRPKPQP
Tissue Specificity Expressed in the immune system. In the kidney, predominantly expressed in the glomerulus, including podocytes.
Function Actin-based motor molecule with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Involved in clathrin-mediated endocytosis and intracellular movement of clathrin-coated vesicles. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14.
Protein Name Unconventional Myosin-Ie
Myosin-Ic
Unconventional Myosin 1e
Cellular Localisation Cytoplasm
Cytoskeleton
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Cell Junction
Colocalizes With F-Actin
In Cultured Podocytes
It Localizes Close To And Is Associated With The Cytoplasmic Membrane
With Enrichment At The Lamellipodia Tips
Colocalizes With Cytoplasmic Vesicles
Including Endocytic Clathrin-Coated Vesicles
Colocalizes With Dynamin At Cytoplasmic Vesicles
Alternative Antibody Names Anti-Unconventional Myosin-Ie antibody
Anti-Myosin-Ic antibody
Anti-Unconventional Myosin 1e antibody
Anti-MYO1E antibody
Anti-MYO1C antibody

Information sourced from Uniprot.org

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