| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MSL2 |
| Gene ID: |
55167 |
| Uniprot ID: |
MSL2_HUMAN |
| Immunogen Region: |
328-577 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 328-577 of human MSL2 (NP_060603.2). |
| Immunogen Sequence: |
CTAATPKIAKLNRKRSRSES DSEKVQPLPISTIIRGPTLG ASAPVTVKRESKISLQPIAT VPNGGTTPKISKTVLLSTKS MKKSHEHGSKKSHSKTKPGI LKKDKAVKEKIPSHHFMPGS PTKTVYKKPQEKKGCKCGRA TQNPSVLTCRGQRCPCYSNR KACLDCICRGCQNSYMANGE KKLEAFAVPEKALEQTRLTL GINVTSIAVRNASTSTSVIN VTGSPVTTFLAASTHDDKS |
| Function | Non-catalytic component of the MSL histone acetyltransferase complex, a multiprotein complex that mediates the majority of histone H4 acetylation at 'Lys-16' (H4K16ac), an epigenetic mark that prevents chromatin compaction. The MSL complex is required for chromosome stability and genome integrity by maintaining homeostatic levels of H4K16ac. The MSL complex is also involved in gene dosage by promoting up-regulation of genes expressed by the X chromosome. X up-regulation is required to compensate for autosomal biallelic expression. The MSL complex also participates in gene dosage compensation by promoting expression of Tsix non-coding RNA. MSL2 plays a key role in gene dosage by ensuring biallelic expression of a subset of dosage-sensitive genes, including many haploinsufficient genes. Acts by promoting promoter-enhancer contacts, thereby preventing DNA methylation of one allele and creating a methylation-free environment for methylation-sensitive transcription factors such as SP1, KANSL1 and KANSL3. Also acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histone H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1. Also involved in the DNA damage response by mediating ubiquitination of TP53/p53 and TP53BP1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Msl2Male-Specific Lethal 2-Like 1Msl2-Like 1Male-Specific Lethal-2 HomologMsl-2Male-Specific Lethal-2 Homolog 1Ring Finger Protein 184 |
| Database Links | Reactome: R-HSA-3214847 |
| Cellular Localisation | NucleusChromosomeAssociates With Both Promoters And Enhancers Of Target GenesMaintaining Promoter-Enhancer Contacts |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Msl2 antibodyAnti-Male-Specific Lethal 2-Like 1 antibodyAnti-Msl2-Like 1 antibodyAnti-Male-Specific Lethal-2 Homolog antibodyAnti-Msl-2 antibodyAnti-Male-Specific Lethal-2 Homolog 1 antibodyAnti-Ring Finger Protein 184 antibodyAnti-MSL2 antibodyAnti-KIAA1585 antibodyAnti-MSL2L1 antibodyAnti-RNF184 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
