• Western blot analysis of extracts of various cell lines, using MSH5 antibody (STJ112160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MSH5 antibody (1-260) (STJ112160)

SKU:
STJ112160

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MSH5 (1-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSH5
Gene ID: 4439
Uniprot ID: MSH5_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MSH5 (NP_751897.1).
Immunogen Sequence: MASLGANPRRTPQGPRPGAA SSGFPSPAPVPGPREAEEEE VEEEEELAEIHLCVLWNSGY LGIAYYDTSDSTIHFMPDAP DHESLKLLQRVLDEINPQSV VTSAKQDENMTRFLGKLASQ EHREPKRPEIIFLPSVDFGL EISKQRLLSGNYSFIPDAMT ATEKILFLSSIIPFDCLLTV RALGGLLKFLGRRRIGVELE DYNVSVPILGFKKFMLTHLV NIDQDTYSVLQIFKSESHP
Tissue Specificity Widely expressed, with high levels in testis and ovary, including granulosa cells. Also expressed in fetal ovary and adrenal gland.
Function Involved in DNA mismatch repair and meiotic recombination processes. Facilitates crossovers between homologs during meiosis.
Protein Name Muts Protein Homolog 5
Hmsh5
Database Links Reactome: R-HSA-912446
Alternative Antibody Names Anti-Muts Protein Homolog 5 antibody
Anti-Hmsh5 antibody
Anti-MSH5 antibody

Information sourced from Uniprot.org

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