| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MMS19 (761-1030) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MMS19 |
| Gene ID: |
64210 |
| Uniprot ID: |
MMS19_HUMAN |
| Immunogen Region: |
761-1030 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 761-1030 of human MMS19 (NP_071757.4). |
| Immunogen Sequence: |
FAGLLNKHPAGQQLDEFLQL AVDKVEAGLGSGPCRSQAFT LLLWVTKALVLRYHPLSSCL TARLMGLLSDPELGPAAADG FSLLMSDCTDVLTRAGHAEV RIMFRQRFFTDNVPALVQGF HAAPQDVKPNYLKGLSHVLN RLPKPVLLPELPTLLSLLLE ALSCPDCVVQLSTLSCLQPL LLEAPQVMSLHVDTLVTKFL NLSSSPSMAVRIAALQCMHA LTRLPTPVLLPYKPQVIRA |
| Tissue Specificity | Ubiquitously expressed with higher expression in testis. |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination by MAGEF1-NSMCE1 ubiquitin ligase complex leading to proteasomal degradation. |
| Function | Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur (Fe-S) cluster assembly into ERCC2/XPD. Together with CIAO2, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery. |
| Protein Name | Mms19 Nucleotide Excision Repair Protein HomologHmms19Met18 HomologMms19-Like Protein |
| Database Links | Reactome: R-HSA-2564830 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindleMicrotubule Organizing CenterCentrosomeIn MitosisEnriched On Centrosomes During ProphaseLocalizes To The Spindle During Metaphase And Surrounds Compacted Spindle Midzone Microtubules During Telophase |
| Alternative Antibody Names | Anti-Mms19 Nucleotide Excision Repair Protein Homolog antibodyAnti-Hmms19 antibodyAnti-Met18 Homolog antibodyAnti-Mms19-Like Protein antibodyAnti-MMS19 antibodyAnti-MMS19L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
